Movement of human cancer cells containing fluorescent Hsp27 in culture before heat shock

The movie BeforeHS of non heat-shocked human SW480-fHsp27 cells was originally published as Supplement 2 to the following article: The role of Hsp27 and actin in the regulation of movement in human cancer cells responding to heat shock. Bindi M. Doshi, Lawrence E. Hightower, Juliet Lee. Cell Stress & Chaperones (2009) 14:445-457. copyright Cell Stress Society International

Movement of human cancer cells containing fluorescent Hsp27 in culture after heat shock

The movie AfterHS was originally published as Supplement 1 in the same article as the BeforeHS movie. This movie was made at 3 h after heat shock of human SW480-fHsp27 cells. Note the increased lamellar dynamics compared with non heat-shocked cells shown in the Before HS movie and the location of the GFP-fHsp27 proteins. copyright Cell Stress Society International

Rat1-control cells expressing H2A-GFP after HS treatment

 

Movie of Rat1-control cells expressing H2A-GFP after HS treatment. Rat1-control cells were transfected with H2A-GFP and then heated for 45 min at 43.5°C before their analysis by live imaging. The movie starts 1 h after HS treatment and covers a period of 24 h post-HS (one image every 30 min). All the nuclei present a deformed morphology. Two apoptotic events occur (one in the middle of the field and a second in the top right of the field after a cell division). copyright Cell Stress Society International Originally published as Supplement 2 in the article titled “The mechanism whereby heat shock induces apoptosis depends on the innate sensitivity of cells to stress.” Kerstin Bellmann & Steve J. Charette & Philippe J. Nadeau & Dominic J. Poirier & Anne Loranger & Jacques Landry. Cell Stress and Chaperones (2010) 15:101113 DOI 10.1007/s12192-009-0126-9

Rat1-myc cells expressing H2A-GFP after HS treatment

 Movie of Rat1-myc cells expressing H2A-GFP after HS treatment. Rat1-myc cells were transfected with H2A-GFP and then heated for 45 min at 43.5°C before their analysis by live imaging. The movie starts 1 h after HS treatment and covers a period of 24 h post-HS (one image every 30 min). All the nuclei present a deformed morphology and then the cells die by apoptosis. Copyright Cell Stress Society International
Originally published as supplement 3 in the paper titled “The mechanism whereby heat shock induces apoptosis depends on the innate sensitivity of cells to stress.” Kerstin Bellmann & Steve J. Charette & Philippe J. Nadeau & Dominic J. Poirier & Anne Loranger & Jacques Landry. Cell Stress and Chaperones (2010) 15:101113 DOI 10.1007/s12192-009-0126-9